Gayle graduated from the University of Cambridge in 2002 and then undertook a rotating internship and residencies in large animal internal medicine and critical care at the Royal Veterinary College. She then moved to the University of Nottingham to undertake an equine cardiology PhD and stayed on staff for 15 years. She then joined IVC Evidensia where she led veterinary professional development and was an equine medicine consultant at Pool House Equine Hospital. She has recently returned to specialty practice as a clinician and educator at Equine Vet Medicine Referrals.